c-kit与p27蛋白在胃肠道间质瘤诊治中的临床意义

Expression and clinical significance of p27 protein and c-kit protein in gastrointestinal stromal tumors

目的研究c-kit和p27蛋白在胃肠道间质瘤中的表达及其与间质瘤临床病理特征的关系,为GIST诊断、预后评估提供客观指标。方法收集54例手术切除的胃肠道间质瘤标本,同时取距瘤灶>5cm的正常组织,应用免疫组织化学S-P检测肿瘤组织及正常组织中c-kit和p27蛋白的表达。结果54例胃肠道间质瘤中,c-kit和p27蛋白阳性表达率分别为94.44%和59.26%。c-kit蛋白表达水平在GIST不同分化程度、有无复发及远处转移的差异无显著性意义(P>0.05),p27蛋白表达水平在GIST不同分化程度、有无局部复发间差异有显著性(P<0.01)。结论c-kit作为特异敏感的标志物,对GIST的诊断及鉴别诊断有重要意义,而检测p27蛋白可作为评估GIST恶性程度和判断预后的重要指标。

Objective To investigate the expression of c-kit and p27 protein in gastrointestinal stroreal tumors（GIST）, and discuss the relationship between their expression and clinicopathological characteristics. Methods lmmunohistoehemical staining was used to detect the expression of c-kit and p27 in 54 cases of GIST and corresponding normal tissues. Results In 54 cases GIST, the expression of c-kit and p27 protein were 94.44 % and 59.26 % respectively. The expression level of c-kit was not found to be correlated with the degree of differentiation, tumor recurrence and distant metastasis （P〉0.05）. The low level expression of p27 protein was inversely correlated with degree of differentiation and tumor recurrence of GIST（P 〈0.01 ）. Conclusions These data shows that the expression of c-kit is an important factor for diagnosis and differential diagnosis of GIST, and p27 expression is related to the progression of GIST.