适于蛋白质组研究的大豆种子蛋白双向电泳技术的改进

IMPROVEMENT OF TWO-DIMENSIONAL ELECTROPHORESIS OF BEAN SEED PROTEIN FOR PROTEOME ANALYSIS

以大豆种子为材料,通过对样品制备、电泳条件以及染色方法等关键步骤进行改进,在大豆种子蛋白分析研究中获得了质量较高的双向电泳图谱,为大豆种子蛋白质组研究提供一较好的实验方法。

Using soybean seed as experimental material, maps of two--dimensional electrophoresis with high quality were obtained through improving the key procedures, such as sample preparation,electrophoresis conditions and staining methods. This improved 2--DE technique will be served as a basis for further investigation of soybean seed proteome.