草原红牛及其杂种牛群体微卫星DNA遗传多态性研究

Study on Genetic Polymorphism of Microsatellite DNA in Grassland Red Cattle and Its Hybrid Population

运用分子生物学技术,分析了草原红牛、草原红牛与利木赞杂交后代群体在8个微卫星座位的遗传多态性。结果表明:在草原红牛中,ETH225,IDVGA2,IDVGA46,IDVGA44多态信息含量分别为0.542 0,0.673 6,0.521 8,0.575 0,这4个座位为高度变异座位。另4个座位BM2113,BM1824,IDVGA55,TGLA44多态信息含量分别为0.369 8,0.360 4,0.353 8,0.470 8,属于中度多态性座位。在杂种牛中,这8个座位的多态信息含量(PIC)均大于0.500 0,属于高度多态性座位。

In this study, the genetic polymorphism of Grassland Red Cattle（GLRC） and its improved hybrid by Limousine（LM）in 8 Microsatellite loci were analyzed with the techniques of molecular biology. The results showed that in the population of Grassland Red Cattle, the polymorphism information content （PIC） in ETH225,IDVGA2, IDVGA46 and IDVGA44 were 0.542 0, 0.673 6, 0.521 8 and 0.575 0 re- spectively. These loci were high polymorphic loci.The PIC in the other 4 loci BM2113,BM1824,IDVGA55 and TGLA44 were 0.3698,0.3604, 0.353 8 and 0.470 8 respectively. They were middle polymorphic loci. In the population of hybrid, the PIC of all loci was more than 0.5.All of the 8 loci belonged to high polymorphic loci.