改良聚丙烯酰胺凝胶电泳法测定籼粳分化的同工酶带

Improving polyacrylamide gel electrophoresis to determine the isozyme loci of indica-japonica differentiation in rice

对同工酶聚丙烯酰胺凝胶电泳法进行改进:液氮研磨样品,0.1 mol/L tris-顺丁烯二酸酐(pH=7.0)缓冲液提取,Bio-rad MP3电泳槽(凝胶规格10 cm×7.5 cm×0.5 mm)在120-150 V电泳2-4 h, 得到快速、经济、分辨率高的分析效果.分析了水稻4种同工酶,发现Est-3,Est-4,Est-x和Pox-x4个新的籼粳分化位点,其中Pox-x为一新位点,该位点在籼稻中有1条带,在粳稻中为哑等位基因.

An efficient polyacrylamide gel electrophoresis for studing isozyme has been developed by improving the following aspects： using liquid nitrogen to grind the materials, 0.1 mol/L tris-maleic anhydride buffer system（pH=7.0） to extract isozyme and then using Bio-rad MP3 electrophoresis system （the gel form is 10 cm×7.5 cm×0.5 mm） to electrophoresis for 2-4 h at 120-150 V. 4 new indica-japonica differentiation locies（Est-3, Est-4, Est-x and Pox-x） of 4 isozymes are discovered in indica and japonica subspieces of rice. The pox-x is a new locus, which has a band in indica subspieces and a null allele in japonica subspieces.