人卵巢癌组织中Tiam1和Rac1的表达与肿瘤侵袭转移的关系

The Expression and Significance of Tiam1 and Rac1 in the Human Ovarian Carcinoma Tissues and Its Correlation to Tumor Metastasis

目的:检测Tiam1和Rac1蛋白在人卵巢癌组织中的表达,探讨其与卵巢癌组织学类型、临床分期、病理分级和侵袭转移的关系。方法:采用免疫组织化学SP法和半定量方法检测Tiam1和Rac1蛋白在52例卵巢癌、12例良性卵巢肿瘤及14例正常卵巢组织中的表达。结果:Tiam1和Rac1蛋白在卵巢癌组织中的阳性表达率分别为80.77%和78.00%,明显高于正常卵巢组织和良性病变(P<0.01);在不同组织学类型和年龄中表达差异无统计学意义(P>0.05);Ⅰ～Ⅱ期阳性表达率为61.53%和46.15%,明显低于Ⅲ～Ⅳ期87.18%和89.74%,(P<0.05);随组织分化程度的降低,二者的染色强度有逐渐增高的趋势,Ⅱ级和Ⅲ级阳性表达率明显高于Ⅰ级(P<0.05);有转移者阳性表达率90.63%和96.87%明显高于无转移者65.00%和50.00%,两者差异有显著性(P<0.05)。结论:Tiam1和Rac1蛋白的表达与临床分期、分化程度及肿瘤转移等密切相关,提示Tiam1和Rac1信号通路在人卵巢癌侵袭转移中起重要作用。

Objective： To investigate the expression of Tiam1（T lymphoma invasion and metastasis inducing protein 1） and Rac1 in human ovarian carcinomas and to explore the correlation between the expression of Tiam1 and Rac1 and the malignant status, including histological types, clinical stage, pathological grade, and metastasis. Methods： Immunohistochemistry （SP method） and semiquantitative analysis were used to measure the expression of Tiam1 and Rac1 in 52 cases of human ovarian carcinomas, 12 cases of human benign ovarian tumors and 14 cases of human normal ovaries. Results： Tiam1 and Rac1 protein was expressed in 80.77% and 78.00% of patients with human ovarian carcinomas respectively, whose expression was higher than those in human benign ovarian tumors and human normal ovaries, suggesting that the difference reached statistical significance （P〈0.05）. There was no obvious difference in the expression of Tiam1 and Rac1 among the histological types and years of age of human ovarian carcinomas. The positive expression of Tiam1 and Rac1 in clinical stage Ⅲ and Ⅳ （87.18% and 89.74%）, as well as with metastasis （90.63% and 96.87%）, were higher than those in clinical stage Ⅰ and Ⅱ （61.53% and 46.15%）, as well as with non-metastasis （65.00% and 50.00%）. The staining intensity of Tiam1 and Rac1 increased gradually with the decrease of the degree of differentiation and the positive expression rate for both Tiam1 and Rac1 in pathological grade Ⅱ and Ⅲ were significantly higher than in pathological grade Ⅰ . Conclusion： The expression of Tiam1 and Rac1 in human ovarian carcinomas is correlated with the clinical stage, the degree of differentiation and tumor metastasis. Tiam1/Rac1 signaling may play a positive role in tumor cell invasion and metastasis.