枯萎病菌的PCR-RFLP分子检测和早期诊断

Molecular detection and early diagnosis of Fusarium with PCR-RFLP program

采用自行设计的、能对镰刀菌18srDNA进行特异性扩增的1对引物,利用PCR-RFLP对尖孢镰刀菌以不同方式侵染的植株进行跟踪检测。结果表明,应用PCR-RFLP检测程序可检测出处于侵染初期和潜育期的枯萎病菌,说明PCR-RFLP能够快速、灵敏、准确地对枯萎病进行早期诊断。

A pair of primer designed personally was used to amplify 18s rDNA of Fusarium and plants inoculated by Fusariurn oxysporum in different ways were followed by PCR-RFI.P, It showed that PCRRFLP programs can detect Fusarium in initial penetration and incubation periods ,which showed that PCRRFLP can diagnose early symptoms of Fusarium wilt disease rapidly,sensitively and exactly.