药用植物材料混配抑制草莓重茬病菌的初步研究

The inhibiting efficacy of medicinal herb mixtures against pathgens of strawberry replanting disease

采用生长速率法,研究了Ds-100,Ds-117和Ds-148 3种药用植物材料按不同比例混配对草莓重茬病主要病原真菌——立枯丝核菌和尖孢镰刀菌的抑菌效果。结果表明,在供试的T15- ,T15- ,T15- ,T15- 和T15- 5种不同混配比例中,T15- 对立枯丝核菌和尖孢镰刀菌的抑菌率分别为85.5%和32.7%,并且在P=0.01水平下,其对立枯丝核菌的抑制作用与其他4种配比的抑菌率差异极显著,对尖孢镰刀菌的抑制作用与T15- ,T15- 和T15- 3种配比的抑菌率差异极显著。采用生长速率法,测定了T15- 不同稀释倍数对立枯丝核菌和尖孢镰刀菌的抑制作用,结果表明:T15- 稀释200,400,800倍对立枯丝核菌的抑菌率分别为70.8%,49.2%,22.1%;对尖孢镰刀菌的抑菌率分别为19.2%,13.3%,7.9%,明显低于对立枯丝核菌的抑制率。此外,测定发现T15- 对立枯丝核菌的EC50为2.2 mg/mL,T15- 稀释50,100,200倍对尖孢镰刀菌孢子萌发的抑菌率分别为59.9%,37.6%和17.0%。

The inhibiting efficacy of five mixtures made by three medicinal plants materials in different proportions were tested to Rhizoctonia solani and Fusarium oxysporum ,the causing agents of strawberry replanting diseases. Results showed that T15- Ⅰ significantly inhibited the mycelial growth of Rhizoctonia solani compared with the other mixtures. The inhibitive rate against the mycelial growth of Rhizoctonia solani 85.5% ,while the inhibitive rate against the mycelial growth of Fusarium oxysporum was 32.7% ,significantly different with the three other mixtures. T15- Ⅰ was tested aganist Rhizoctonia solani and Fusarium ocrysporum in different dilutions. The results showed that the suppressive effect of T15- Ⅰ when diluted to 0.5%,0.25% and 0. 125% on Rhizoctonia solani and Fusarium oxysporum were 70.8%,49.2%,22.1% and 19. 2%, 13. 3%, 7. 9% respectively. Besides, for Rhizoctonia solani, the EC50 of T15- Ⅰ was 2. 2 mg/mL. The inhibitive rates against conidial germination of Fusarium oxysporum were respectively reached 59.9%,37.6% and 17.0% when diluted to 2%, 1% and 0.5%.