山西省乙型肝炎病毒基因型分布及临床意义研究

Distribution of hepatitis B virus genotypes in Shanxi province and its significance

目的了解山西省乙型肝炎病毒(HBV)基因型流行病学分布特征,并分析不同基因型与HBV感染谱的相关性。方法用S基因巢式聚合酶链反应-限制片段长度多态性(PCR-RFLP)的基因型分型方法,对44例携带者、70例慢性乙型肝炎、34例慢性重症乙型肝炎、29例肝硬化患者感染的HBV进行分型,并分析了不同感染谱中HBV基因型的分布。结果①177例感染者HBV基因型C型130例(73.5%)和B型43例(24.3%),偶见D型1例(0.6%),未定型3例(1.7%)。②各种乙型肝炎患者的HBV基因型以C型为主,HBV携带者的基因型主要为B基因型。结论山西省乙型肝炎病毒感染者以C型和B型为优势基因型,与我国北方地区HBV基因型分布相似,但亦有少数其他基因型存在。HBV基因型C可能是乙型肝炎病情进展的相关因素。

Objective To understand the distribution of hepatitis B virus genotypes in Shanxi province and to analyse the relationship between HBV genotypes and patients with hepatitis B. Method A nested polymerase chain reaction （nested-PCR） and restriction fragment length polymorphism （RFLP） analysis assay amplifying S-segment sequences of HBV DNA were used for analyzing the distribution of HBV genotypes among 44 HBV carriers,70 patients with chronic hepatitis B, 34 patients with severe chronic hepatitis B and 29 cases liver cirrhosis. Result The following distribution of genotypes was found： HBV genotype C was 73.5%, genotype B was 24.3%, and D-genotype was 0.6%. 3 cases failed to be detected. The genotype C seemed to be most common in patients with chronical active hepatitis and liver cirrhosis.Conclusion The genotypes C and B are two predominant genotypes in Shanxi province, genotype C may be associated with the development of hepatitis.