摘要
Objective To screen the coaggregating pairs between perio-pathogenic and cariogenic bacteria and to investigate the susceptibility of these coaggregations to inhibitors. Methods 4 strains of perio-pathogenic bacteria, Fusobacterium nuleatum (Fn) ATCC 10953, Actinobacilllus actinomycetem comitans (Aa) Y4, Porphyromonas gingivalis (Pg) ATCC 33277,Prevotella intermedia (Pi) ATCC 25261 and 4 strains of cariogenic bacteria, Streptococcus mutans (Sm) lngbritt, Streptococcus sanguis (Ss) 34, Actinomyces viscosus ( Av) 19246 and Lactobacillus acidophilus (La) ATCC 4356 were used to determine the coaggregating degrees of various combinations of the above bacteria by a visual assay and a turbidimetric assay. Then more than + 2 ( or 20% ) coaggregation degrees' pairs were used to investigate the inhibitory effect of lactose and arginine and to identify the minimum of their coaggregation-inhibitory concentration. Results The coaggregation degrees of Fn-Av, Pg-Av, Fn-Sm, Fn-Ss, Fn-La and Pg-Ss pairs were higher than + 2 ( 20% ). 3.0 - 6.0mmol/L of arginine were considerably effective to the above pairs except Fn-Av pair and the disaggregation degrees were 49% - 92%. The maximum of their disaggregation degree to Fn-Av pair was just 18%. 120 - 300mmol/L of lactose were significantly effective to Pg-Ss pair, the disaggregation degrees were 57% - 91%. They partially inhibited Pg-Av pair and were almost ineffective to FnG^+pairs. Conclusion The coaggregations between perio-pathogenic and cariogenic bacteria are highly specific. Most of them are relatively sensitive to arginine.
基金
SupportedbygrantfromNaturalScientificFoundation(30070813)ofChina.