微小病毒TLMV的检测研究

On Detecting Mini Virus TLMV

目的 TLMV是一种新型与输血性肝炎相关的人类DNA病毒,检测中国慢性乙型肝炎患者体内是否存在TLMV感染.方法根据日本学者Shunji Mishiro提供的序列选择TLMV-CBD279和CBD231最保守区设计引物,用慢性乙型肝炎且TTV阳性患者血清制备DNA模板,进行PCR扩增;将阳性PCR产物连接至pGEM(r)T质粒,碱裂解法提取质粒DNA并纯化.以T7引物为起始全自动荧光测序仪测序.结果获得了162bp基因序列,该序列与日本株CBD231,CBD279同源性为72%.结论说明在中国慢性乙型肝炎患者体内存在TLMV感染.

Objective TTV-like mini virus（TLMV） is a newly identified human virus which is transmitted by blood-transfusion and associated with liver disease. Methods Since it was first reported in Japan in 2000 by Shunji Mishiro, we designed two sets of primers in the most conservative regions of Japan strains TLMV- CBD279 and CBD231 to amplify TLMV templete extracted from sera of patients with chronic hepatitis B using PCR. PCR products were cloned to pGEM-T vector and sequenced. Results The results showed that 72% of TLMV sequence isolated in China is identical to that in Japan. Conclusion There are TLMV infection in patients with chronic hepatitis B in China.