摘要
主要介绍了RFLP、RAPD、SSR、AFLP等四种分子标记技术的原理、方法、特点及其在花生上的应用现状:①RAPD、SSR、AFLP都可以分析花生品种的多样性;②RAPD技术可以对杂交后代进行鉴定;③利用RFLP、RAPD技术绘制花生指纹图谱;④利用分子标记技术研究花生根瘤菌;⑤基于花生的RFLP、RAPD技术的改进。同时,展望了分子标记技术在花生上的应用前景,以期对花生的分子标记育种有所帮助。
In this article, four kinds of molecular marker techniques' (RFLP,RAPD,SSR,AFLP) principles, methods and characters are introduced. We also draw the conclusions from the review as follows: RAPD, SSR and AFLP can be used to analysis the variation of peanut; ①The deep generations of hybridized peanut can be identified by RAPD; ②Use RFLP and RAPD to draw the fingerprinting of peanut; ③The research on Bradyrhizobiumsp Arachis; ④The progress in the technique of RFLP and RAPD based on peanut research. At the end of the article, we prospecting the foreground of the molecular markers used in peanut. We hope this will help the research.
出处
《中国农学通报》
CSCD
2005年第8期36-40,共5页
Chinese Agricultural Science Bulletin
基金
"十五"国家重点科技攻关项目"花生新品种选育研究"(2001BA511B11)