贯叶连翘茎段和叶片的离体培养及植株再生研究

In vitro Culture and Plantlet Regeneration from the Stem and Leaf of Hypericum perforatum

以贯叶连翘的叶片和茎段作为外植体,培养在含有不同种类及浓度激素的MS基本培养基上,进行愈伤组织、丛生芽、根的诱导实验,并进行试管苗的移栽。结果表明,2,4-D有利于贯叶连翘愈伤组织的形成;加有6-BA0.5mg/L的MS培养基最适合丛生芽的形成;诱导生根较好的培养基是MS+IBA0.5mg/L+NAA0.5mg/L和MS+IBA1.0mg/L。

The stem and leaf of the Hypericum perforatum were used as explants, and cultured in basic MS medium supplemented with different kinds and concentrations of hormones. The callus, the buds and roots were induced, and cultivation of the tube seedling were carried out. Results have shown, the callus is easy to be induced when MS medium is supplemented with 2,4-D; MS medium supplemented with 6-BA 0.5 mg/L is most suitable for the formation of buds; MS medium supplemented with IBA 0.5 mg/L and NAA 0.5 mg/L, and MS with IBA 1.0 mg/L are effective in promoting the rooting of Hypericum perforatum.