Photofrin-Herceptin偶联物对肿瘤细胞株的体外杀伤效应

Killing effect of Photofrin-Herceptin immunoconjugate on tumor cell lines in vitro

目的研究Photofrin-Herceptin偶联物对HER-2表达阳性肿瘤细胞的特异性杀伤作用,为进一步的光免疫治疗奠定基础。方法用碳二亚胺(EDCI)将光敏剂Photofrin与单抗Herceptin偶联,并分别对SK-BR-3、MCF-7、A549这3种HER-2表达程度不同的肿瘤细胞进行体外杀伤实验,用MTT法检测。结果偶联物的免疫活性较Herceptin降低,但其对HER-2表达阳性的肿瘤细胞的杀伤作用较Photofrin、Herceptin及Herceptin与Photofin混合物明显增强(P<0.05),对于HER-2表达阴性的肿瘤细胞杀伤效果无明显差异。结论Photofrin-Herceptin偶联物可特异性杀伤HER-2表达阳性的肿瘤细胞。

Objective To study the specific killing effect of the Photofrin-Herceptin immunoconjugate on the tumor cells expressing HER-2. Methods Photofrin （porfimer sodium） was covalently coupled to Herceptin （trastuzumab） via [1-ethyl-3- （3-dimethyl-aminopropyl）carbodiimide hydrochloride]（EDCI）, and the killing effect of this conjugate was evaluated by means of MTT assay on SK-BR-3, MCF-7 and A549 cell lines expressing HER-2 at different levels. Results The conjugate had weaker immunoactivity than Herceptin, but possessed stronger killing effect on the cell lines expressing HER-2 than Photofrin, Herceptin, as well as the mixture of Photofrin and Herceptin （P〈0.05）. In the cell lines negative for HER-2 expression, the killing effect of the conjugate was similar to that of Photofrin, Herceptin, and their mixture. Conclusion Photofrin-Herceptin immunoconjugate can specifically kill HER-positive cells in vitro.