树突状细胞密度、p53表达在进展期胃癌中的临床意义

Clinical significance of dendritic cell infiltration and expression of p53 in advanced gastric adenocarcinoma

目的研究进展期胃癌树突状细胞浸润和p53表达、临床病理学特性之间的相关性及其临床意义。方法用免疫组化法使用通过图像分析仪检测50例进展期胃癌细胞中树突状细胞标志蛋白S-100及p53表达。结果50例肿瘤患者中树突状细胞均值为(10.160±7.939)/mm2。树突状细胞浸润密度在有淋巴结转移的胃癌组低于无淋巴结转移的胃癌组(P=0.015)。有淋巴管侵犯的胃癌组中树突状细胞浸润密度低于无淋巴管侵犯的胃癌组(P=0.011)。p53表达阳性的胃癌组树突状细胞浸润密度低于p53表达阴性的胃癌组(P=0.032)。50例患者5年生存率中,树突状细胞高浸润组(≥10.16/mm2)明显好于树突状细胞低浸润组(<10.16/mm2)(P=0.001)。树突状细胞高浸润、p53阴性表达者预后最好。结论肿瘤内和肿瘤周围树突状细胞浸润可代表宿主局部免疫状态。较少程度的树突状细胞浸润、p53阳性表达可促进疾病的进展,与较差的预后相关。

Objective To investigate the correlation between dendritic cell infiltration , the clinicopathologie characters and p53 expression in advaned gastric carcinoma. In addition, the prognostic significance of dendritic cell infiltration and p53 expression was evaluated. Methods The S - 100 expression of markd protein of dendritic cells and expression of p53 in the tumor were detected by immtmohistochemical method using image analysis system. Results The DCs mean of the 50 tumors was （10. 160± 7.939）/mm^2. The numbers of dendritic cell infiltration in the lymph node metastasis groups were lower than that of without lymph node metastasis groups （P = 0.015）. The DCs in gastric cancer groups with lymphangial invasion were lower than that of without lymphangial invasion. The DCs in gastric cancer groups with the positive expression of p53 were low as compared with that of the negative expression of p53. In the 5 - year survial rate of 50 patients, the groups who had tumors with high DCs （≥ 10.16/mm^2 ） was significanly better than the groups who had tumors with low DCs （ 〈 10.16/mm^2 ） （ P = 0.00! ）. The prognosis of the groups with high DCs and negative expression of p53 was the best of all groups. Conchsion The infiltration of dendritic cells in and around tumor represented the local immune status of the host. Less extensive dendritic cell infiltration and positive expression of p53 may contribute to disease progression and could be correlated with a poorer prognosis.