促红细胞生成素对脑缺氧的保护作用

Effect of erythropoietin on neuron death induced by hypoxia

目的在细胞水平探讨促红细胞生成素(EPO)对缺氧诱发的神经元损伤的改善作用及缺糖缺氧诱发的脑片损伤的改善作用。方法 采用原代新生大鼠皮层培养和小鼠脑片的方法,分别以形态学和二苯基四氮唑溴盐(MTT)及2,3,5-三苯基氯化四氮唑(TTC)染色为指标观察神经元及脑片的损伤和药物的改善作用。结果EPO在10-11,10-12mol·L-1能显著改善缺氧24 h引起的培养神经元死亡,其在10-9mol·L-1能改善缺氧45min或缺糖缺氧15min引起的脑片梗死。结论EPO对缺氧诱发的神经元死亡和缺糖缺氧诱发的脑片梗死具有保护作用。

OBJECTIVE To determine the effect of erythropoietin on neuron death induced by hypoxia and on hypoxic-ischemic brain slice injury, METHODS The primary cortical cell culture and brain slice were adopted. Neuron morphology, MTT and TTC staining were used to investigate the effects of erythropoietin. RESULTS Erythropoietin at the doses of 10^-11 and 10^-12 mol· L^-1 reversed the neuron death induced by hypoxia for 24 h. Erythropoietin at the doses of 10^-9 mol· L^-1 reversed the brain slice injury, induced by hypoxia for 45 min or with oxygen-glucose diprivation for 15 min. CONCLUSION Erythropoietin reduces neuron death and brain slice injury indueed by hypoxia or with glucose diprivation.