中国北方蒙古族、汉族、达斡尔族人群的ACE基因的多态性

The polymorphism in intron 16 of ace in the populations of Chinese Mongolians,Hans and Daurs

目的调查蒙古族、汉族、达斡尔族人群的ACE基因I/N的多态性,分析这些民族的遗传特点。方法应用ACE基因内含子的16多态部位侧翼的前体进行PCR扩增。等位基因在琼脂胶上电泳,溴乙啶染色,紫外灯下观察。结果蒙古族人群的DD,DI和Ⅱ基因型的频率是0.51,0.26和0.23;汉族的是0.33,0.32和0.35;达斡尔族人群的是0.26,0.60和0.13。结论ACE基因I/N的多态性在蒙古族、汉族和达斡尔族三民族的分布是不同的。

Objective ： To investigate the Insertion/Deletion （I/D ） Polymorphism ot anglotensm convening enzyme （ACE） in mongonan, Hans and Daur population, and analysis their genetics characteristics. Methods ： Genomic DNA was amplified by polymorphic region in intron 16 of the ACE gene. Allele were deeeeted on agarose gel stained with ethidium bromide. Results：The frequencies of DD,DI and Ⅱ genotype were 0.51 , 0.26 and 0.23 in Mongolian; and 0.33,0.32 and 0.35 in the Hans ; 0.26,0.60 and 0.13 in Daurs respectively. Conclusion： The polymorphism of ACE gene are diffierenees in three nations.