酶解鱼鳞胶制备小分子多肽的研究

Preparation of Low-molecular Weight Peptide from Scale by Enzymatic Hydrolysis

以鱼鳞为原料采用蛋白酶酶解,确定制备小分子多肽的最佳工艺条件。发现用混合蛋白酶进行阶段酶解效果最好;对两种脱苦、脱腥的方法进行了比较,发现用复配脱色剂比用活性碳优越;发现超滤分离技术和反渗透膜分离技术对水解液有纯化、浓缩作用;最后经微胶囊化包埋喷雾干燥等技术得到的粉末制品含有较多的小分子多肽,是医药和食品的良好中间体产品。

The best technology of preparing low-molecular weight peptide from scale by enzymatic hydrolysis has been introduced. Using mixed enzyme is better than other enzymes. Tow methods of removing fishness and bitlemess are compared, which shows that adding compound decolorer is better than andding pure carbon. Ultrafiltrafion and reverse osmosis membrane sepatafion technique can sublimate and concentrate the hydrolyzed liquor. The product is a kind of healthy food which contains many low-molecular weight peptides.