金叶败毒制剂抑制人巨细胞病毒蛋白激酶pul97的实验研究

Experimental Study on Effect of Jinye Baidu Preparation in Inhibiting Human Cytomegalovirus Protein Kinase pul 97ST

目的研究中药金叶败毒制剂对人巨细胞病毒(humancytomegalovirus,HCMV)蛋白激酶pul97的抑制作用,探讨其治疗HCMV感染的分子机制。方法采用半定量RT-PCR方法,检测中药金叶败毒制剂和更昔洛韦(GCV)干预后感染细胞HCMVpul97mRNA表达水平,应用MTT法观察两种药物对感染细胞的增殖活性的影响。结果金叶败毒制剂和更昔洛韦对HCMVpul97mRNA的表达有明显的抑制作用,在HCMV感染72h后两种药物均可明显增强感染细胞的增殖活性。结论金叶败毒制剂可能通过抑制HCMV蛋白激酶pul97基因的表达而抑制HCMV的表达和复制,从而促进感染细胞的增殖而达到抗病毒作用。

Objective To study the inhibitory effect of Jinye Baidu Preparation （JBP）, a Chinese medicinal preparation, on human cytomegalovirus protein kinase pul97 and to explore its molecular mechanism in treating human cytomegalovirus （HCMV） infection. Methods Expression of the HCMV pul97mRNA in infected cells was measured by semi-quantitative RT-PCR before and after intervention of JBP or Ganciclovir （GCV）, and effect of the two medicines on the proliferation activity of the infected cells was observed by MTT. Results Both JBP and GCV showed obvious inhibitory action on HCMV pul97mRNA. They could significantly enhance the proliferation activity of the cells 72 hours after HCMV infection, Conclusion JBP could inhibit the gene expression and duplication of HCMV by inhibiting the gene expression of HCMV protein kinase pul97 to enhance the proliferation activity of the infected cells so as to achieve its anti-virus action.