ADAMs参与哺乳动物精-卵结合与融合

ADAMs involved in mammalian sperm-egg plasma membrane adhesion and fusion

ADAMs家族是含多结构域的跨膜蛋白。睾丸特异的ADAMs,在精子发生与附睾精子转运过程中,经过蛋白水解成为成熟精子的分子形式,与精-卵质膜结合和融合有关。对于精-卵质膜相互作用,ADAMs去整合素域具有关键氨基酸残基和特殊模体。模拟ADAM2和ADAM3去整合素域的短肽能用于鉴别特异性卵子识别蛋白。精子ADAMs去整合素域与卵子膜蛋白整合素β1、α4/α9、α6和CD9相互作用,介导了精卵质膜的结合与融合。

The ADAMs （A disintegrin and A metalloprotease）family are multidomain proteins.Some ADAMs appear to be testis-specific and have been shown to be proteolytically to form functional moleculars on mature sperm during spermatogenesis and epididymal transport. The testis-specific ADAMs may play a role in spermegg plasma membrane adhesion and fusion. It is necessary that the disintegrin domain of ADAMs contain some key amino acids and specific motif for sperm-egg interaction. The short synthetic peptides, based on the disintegrin-like domains of ADAM2 and ADAM3 can be used in identifying specific oocyte recognition proteins. It has been suggested that the disintegrin domain of sperm ADAMs can interact with integrinsβ1,α4/α9,α6, and β1 integrin-associated protein CD9 on the egg.