肌肽在维生素C-亚铁离子系统中对DNA损伤的保护作用

Protective effects of carnosine on DNA oxidant damage in Ascorbate -Fe^(2+) system

目的研究肌肽在维生素C-亚铁离子系统中对DNA氧化损伤的作用。方法采用硫代巴比妥酸法在脱氧核糖水平上检测肌肽对DNA氧化损伤的保护作用。设咪唑、L-组氨酸、β-丙氨酸、甘露醇、苯甲酸钠、二甲基亚砜为对照,比较其抗氧化能力的强弱,并对其抗氧化机制做初步探讨。结果在此实验条件下肌肽的抗氧化能力明显优于苯甲酸钠等常用抗氧化剂,其抗氧化动力学曲线与咪唑相似。结论肌肽在维生素C-亚铁离子系统中对DNA氧化损伤有明显的保护作用,其抗氧化机制可能与其所含的咪唑环有关。

Purpose To study the effect of carnosine in Ascorbate-Fe^2＋ system which can induce DNA oxidant damage. Methods The products of DNA oxidant damage can be monitored by TBA reaction. The antioxidant abilities of carnosine were observed at deoxyribose level and were compared with imidazole, L-histidine,β-alanine, Mannitol, Benzoate and DMSO. Results carnosine has a better protection than mannitol, benzoate, etc. in the Ascorbate-Fe^2＋ system. Its antioxidative curve is similar to that of imidazole. Conclusion Carnosine has a very good protection effects on DNA oxidant damage in Ascorbate-Fe2 ~ system and its antioxidant ability has a close connection with its imidazole cycle.