辅因子再生研究进展

Progresses on cofactor regeneration

许多有价值的酶催化反应都需要辅因子的参与。因为辅因子价格昂贵,所以,在酶催化工业应用中,需要实现辅因子原位再生。经过几十年研究,出现了酶法、化学法、电化学法、光化学法和基因工程法等手段实现烟酰胺类辅因子(NAD(P)H)、ATP、糖核苷酸等辅因子再生。对辅因子再生研究中取得的进展以及存在的问题进行讨论。

Cofactor-dependent enzymes catalyze many useful reactions. Due to the high cost of the cofactors, in situ cofactor regeneration is required for preparative applications. After two decades of research, various cofactors,such as NAD （P）H, ATP, sugar nucleotides can now be regenerated using enzymatic, chemical, electrochemical, photochemical or genetic engineering based methods. The recent advances in cofactors regeneration are reviewed in this paper.