异基因小鼠胚胎胸腺移植治疗红斑狼疮的实验观察

Experimental Observation of Transplantation of Xenogenous Thymus to Mice with Systemic Lupus Erythematousus

目的探讨胚胎胸腺组织移植对系统性红斑狼疮(SLE)模型鼠的治疗效果。方法将21只符合要求的BALB/c×C57BL/6F1小鼠经尾静脉注射亲代淋巴细胞混合悬液后随机分成3(A^C)组,于SLE模型建立成功后接受相应治疗方案,第25天处死模型小鼠,采用流式细胞技术检测各组小鼠外周血中CD4+/CD8+T细胞的比值;电子天平称取小鼠胸腺湿质量后计算胸腺指数;直接免疫荧光法检测肾冰冻切片免疫复合物的荧光强度;间接免疫荧光法检测血清中抗核抗体(ANA)的荧光滴度。结果①C组外周血中CD4+/CD8+T细胞的比值显著高于A组(P<0.05),但A组与B组相比,差异无显著性(P>0.05);②C组胸腺指数与B组相比,无显著性差异(P>0.05),但显著高于A组(P<0.05);③C组肾脏冰冻切片荧光强度及外周血ANA荧光滴度均低于A组和B组(P<0.05)。结论胚胎胸腺移植对SLE模型鼠有显著治疗效果,有望运用于临床SLE的治疗。

Objective To evaluate the therapeutic effects of fetal thymus transplantation to the mice with systemic lupus erythematosus（SLE）. Methods Twenty-one BALB/c×C57BL/6 F1 mice were divided into 3 groups after their being injected with their parental lymphocytes suspended solutions. When the models were established successfully,a therapy by adrenaline was conducted in group A： fetal thymus transplantation was in Group B;and adrenaline and fetal thymus transplantation was in Group C. At 25 days after the therapies,mice serum were detected by flow cytometry（FCM） for T cells ratio of CD4/CD8;Thymus were weighed with an elecronic equiarmhalance, then the indexes of thymus were calculated; Direct immunofluorescence technique was used to test the intensities of fluorescence of mice kidney frozen sections; The serum fluorescence titres of anti-nuclear antibodies（ANA） were tested by indirect immunofluorescence technique. Results①The T cells CD4/CD8 ratios in group C were higher than that in group A（P〈 0.05）, while the ratios did not show any significant difference in group A and group B（P〉0. 05）;②Thymus indexes in group C did not have any significant difference compared with that in group B（P〉0. 05） ,while the indexes in group C was higher than that in group A（P〈0. 05） ;③The fluorescence intensities of mice kidney frozen sections and ANA titres in group C were lower than those in group A and B（P〈0.05）. Conclusion Fetal thymus transplantation perform significant therapeutic effects on SLE mice and it could be used in clinical treatment of SLE.