芦荟大黄素对兔髂动脉损伤后内膜增生及血管重塑反应的在体研究

Effect of Aloe Emodin on Intimal Hyperplasia and Vascular Remodeling after Iliac Arteries' Injury of Rabbits: A Study in Vivo

目的探讨芦荟大黄素对兔髂动脉损伤后内膜增生及血管重塑反应的影响。方法将14只纯种日本大耳白兔随机分为髂动脉球囊损伤用药组和非用药组,于手术前后分别给予芦荟大黄素〔40mg/(kg·d)×7d〕或等体积的生理盐水肌肉注射。14d后,取出左右髂总动脉,用计算机图像分析仪对其切片进行形态测量分析。结果用药组与未用药组比较,最大内膜厚度均值稍低〔(0.043±0.013)mm比(0.048±0.015)mm,P>0.05〕;新生内膜面积减小〔(0.025±0.005)mm2比(0.035±0.008)mm2,P<0.05〕;管腔面积增大〔(0.098±0.011)mm2比(0.08±0.013)mm2,P<0.05〕;管腔面积和内膜面积及外弹力膜面积之比升高(用药组为0.793±0.036、0.617±0.054;非用药组为0.699±0.063、0.538±0.057,P<0.05)。用药组与未用药组比较,外弹力膜面积稍增大〔(0.160±0.010)mm2比(0.152±0.01)mm2,P<0.2〕,但与未受损伤的正常髂动脉外弹力膜面积〔(0.134±0.023)mm2〕相比,差异有统计学意义(P<0.05)。结论芦荟大黄素可以抑制球囊损伤后内膜的增生,并有可能对损伤后的血管重塑产生有利的影响。

Objective To investigate the impact of Aloe emodin on intimal hyperplasia after iliac arteries injury of rabbits and the preventive effect of this agent on restenosis in animal model. Methods Fourteen Japanese white rabbits（weights 1.5 kg） received emodin [40 mg/（kg· d） im] or 0.9 % NaCl 0.5 hours before and 7 d after balloon injury of the right iliac artery. Two weeks later, the rabbits were killed and both the injured right and the uninjured control left iliac arteries were isolated and fixed and embedded in paraffin and serially sectioned （ 10 μm） and stained with Weigert＇ s elastic lamina. The cross-sectional surface areas of the vessels within the external elastic lamina （the total area）, within the lumen （the lumen area）, and within the intimal elastic lamina （the intimal area） were measured. The maximal intimal thickness was also measured with a LeiCA Qwin Image Processing and Analysis System. Results Compared with control, in Aloe-emodin treated groups the maximal intimal thickness were a little smaller [（0. 048 ± 0. 015）mm, （0. 043 ± 0. 013）mm, respectivelyl and the vascular areas were slightly greater [ （0. 160 ±0.010） mm^2, （0. 152 ± 0.01 ） mm^2, respectively ] , but there were no significant differences. The lumen size was significantly greater in Aloe emodin-treated [ （0. 098 ± 0.011 ） mm^2] than in control rabbits [ （0.08 ± 0. 013 ） mm^2, P 〈 0.05], the areas of neointimal and the ratios of neointimal to intimal and vascular areas were significantly less in Aloe emodin-treated [（0.025±0.005）mm^2, 0.793±0.036, 0.617±0.0541 than in control rabbits [（0.035±0.008）mm^2, 0.699±0.063, 0. 538±0. 057, P 〈 0.05]. Conclusion The hypothesis has been proved correct that systemic administration of Aloe emodin immediately before （0.5 h） and after intervention effectively inhibits myointimal proliferation after balloon injury of the rabbit iliac artery in vivo and may be useful in the vascular remodeling. It seems that this agent may be useful in the prevention of coronary restenosis.