护肝片对纤维化肝组织NF-κB表达的抑制作用

EFFECT OF LIVER-AID TABLETS ON NF-κB EXPRESSION IN RAT HEPATIC FIBROSIS

目的探讨护肝片对中、晚期纤维化大鼠肝组织核转录因子-κB(NF-κB)表达的影响。方法采用12·5%CCl4诱导的大鼠肝纤维化模型,自造模之日起,大鼠分组灌胃给药(护肝片921mg·kg-1)或溶媒,每d一次,直至8或13周末,分别处死动物,取左叶肝组织石蜡包埋,制作组织芯片,免疫组化SP法检测NF-κBp65蛋白表达的改变,并用MetaMorph图像分析系统对NF-κBp65蛋白表达量进行定量分析。结果1.模型复制8周和13周,模型组的肝损伤及其纤维化分级均明显高于正常组(P<0·01),护肝片组的肝损伤及其纤维化分级均轻于模型组。2.模型复制8周和13周,模型组NF-κBp65蛋白的表达较正常组均明显增强(P<0·01);3.护肝片显著抑制8、13周纤维化肝组织NF-κBp65蛋白的表达(P<0·01)。结论护肝片可减轻肝组织的损伤及其纤维化程度,抑制NF-κB的表达可能是其抗肝纤维化作用的靶点之一。

Objective To study the effect of Liver-aid tablets on the expression of NF-κB （NF-κB） in rat hepatic fibrosis. Methods SD rats were divided into three groups： normal control, model control and Liver-aid. CCh （12. 5%, 4ml · kg^-1） was injected subcutaneously in model rats twice a week for 8 and 13 weeks. The drug （921mg · kg^-1 in the group of Liver-aid tablets, but 0. 5% CMC-Na in the control groups） was given intragastrically once a day for 8 and 13 weeks. The rats were killed at the end of 8th and 13th week respectively, and the left liver tissue was used to make tissue microarrays （TMA）. The TMA sections were used for Immunohistochemistry （IHC） to detect the protein expression of NF-κB. The results of IHC were quantified by MetaMorph imaging analysis system. Results 1. In 8 and 13 weeks, the degree of liver injury and the grade of fibrosis in the model group were higher than those in the normal group （P〈0. 01 ）, which was ameliorated remarkably by Liver-aid tablets. 2. The protein expression of NF-κB in the model group was stronger than that in the normal group in 8 and 13 weeks （P〈 0. 01）. 3. Liver-aid tablets inhibited the protein expression of NF-κB in the fibrotic liver tissues of rats in 8 and 13 weeks （P〈0. 01）. Conclusion Liver-aid tablets have anti-fibrosis effects on CCl4-induced rat liver fibrosis, which might be achieved by inhibiting the exoression of NF-κB.