肺癌脆性组氨酸三联体基因表达及其与细胞增殖的关系

FHIT EXPRESSION IN LUNG CANCER AND ITS CORRELATION WITH PROLIFERATION

目的探讨脆性组氨酸三联体基因(FHIT)在肺癌中表达及其与肺癌细胞增殖的相关性。方法利用组织芯片和免疫组织化学技术,检测110例肺癌及25例良性病变的肺组织标本FHIT蛋白及Ki-67、P53、P21WAF1蛋白表达。结果(1)良性病变的肺组织FHIT基因异常表达率为16%,肺癌组织异常表达率为85·5%,二者比较,差异有极显著性(χ2=49·390,P=0·000);(2)细胞增殖指数(proliferationindex,PI)不同的肺癌组织中,FHIT基因表达差异有极显著性(χ2=30·145,P=0·000),且FHIT基因表达与PI负相关(r=-0·0418,P=0·000);(3)在P53表达阴性的肺癌组织中,P21WAF1阴性组与P21WAF1阳性组比较,FHIT的表达差异无统计学意义(χ2=4·125,P=0·248)。结论FHIT基因可能参与肿瘤细胞增殖的调控,FHIT基因表达下降可能与肺癌的发生密切相关。

Objective To investigated the incidence of fragile histidine triad （FHIT） gene expression in a series of lung carcinomas and its correlation with cell proliferation. Methods The FHIT, Ki-67, P53, and P21W^WAF1 protein expressions in the specimens of 110 cases of lung cancer and 25 benign lung lesions were detected by immunohistochemistry and tissue microarray technique. Results （1） Abnormal expression of FHIT was 16% in benign lung lesions but 85.5% in lung cancer, with highly significant difference （X^2 =49. 390, P =0. 000）. （2） When the proliferation index （PI） was difference, FHIT expression in the lung carcinoma was very significantly different （X^2 =30. 145, P=0. 000）, and FHIT expression was negatively correlated with PI （r=-0. 0418, P=0. 000）. （3） When P53 expression was negative and the expression level of P21^WAF1 was different, the difference of FHIT expression in the lung carcinoma was not statistically significant （X^2=4. 125, P=0. 248）. Conclusion FHIT gene may play a role in the regulation of cell proliferation of lung cancer, and the reduction in FHIT expression may be related to tile pathogenesis of lung cancer.