辛伐他汀对大鼠骨髓基质细胞分化和矿化功能的影响

Effect of simvastatins on differentiation and mineralization of bone marrow stromal cells in rats

目的:研究辛伐他汀对大鼠骨髓基质细胞分化和矿化功能的影响,探讨其刺激成骨及防治骨质疏松症的机制。方法:体外培养4周龄大鼠骨髓基质细胞,以不同浓度辛伐他汀(10-9mol/l,10-8mol/l,10-7mol/l,10-6mol/l)作用,观察细胞生长情况和形态变化,测定细胞碱性磷酸酶(ALP)活性,I型胶原的分泌和矿化能力。结果:辛伐他汀各浓度组的ALP活性均增加,以10-7mol/l浓度作用最显著(P<0.05);辛伐他汀10-8mol/l和10-7mol/l浓度明显促进I型胶原的分泌;辛伐他汀显著提高细胞的矿化能力,以10-7mol/l浓度作用最显著(+44.5%,P<0.05)。结论:辛伐他汀促进骨髓基质细胞的分化,提高其矿化能力,有助于骨质疏松症的防治。

Objective： To study the effect of simvastatin on differentiation and mineralization of Bone Marrow Stromal Cells and to explore the mechanism of stimulated bone formation and prevention and treatment of osteoporosis by simvastatin. Methods： Bone marrow cells were isolated from four-week-old Spragne-Dawley rats and cultured in DMEM medium, different concentration of simvastatin （10^-9mol/1, 10^-8mol/l, 10^-7mol/l, 10^-6mol/l） on Bone Marrow Stromal Cell were selected. The growth and morphology of cultured cells were observed on a phase-contrast microscope. Alkaline phosphatase activity and Type-Ⅰ pro-collagen of BMSc were detected. Capacity of mineralization was investigated by counting bone nodules. Results： Compared with control group, alkaline phosphatase activity of BMSc was increased in all treatment groups, and its value was significantly higher in 10^-7mol/l group than other treatment groups. Type-Ⅰ pro-collagen level showed a significant increase in 10^-7mol/l and 10^-8mol/l group （P〈0.05） With regard to the capability of mineralization, nodules number was significantly higher in 10^-7mol/l group （＋44.5%, P〈0.05） than in other groups. Conclusion： We confirmed that simvastatin promote differentiation and capacity of mineralization of BMSC and contribute to the prevention and treatment of osteoporosis.