蛋白质分子的定点突变和化学修饰(英文)

A Discussion of Site-directed Mutagenesis Versus Chemical Modification

回顾蛋白质工程研究及应用技术、手段和方法,分析定点突变及化学修饰在改造蛋白质分子结构与功能方面的异同。定点突变技术可以随心所欲地在已知DNA序列中取代、插入或缺失一定长度的核苷酸片段。该方法与使用化学因素导致突变的方法相比,具有突变率高、简单易行、重复性好的特点。

Recent researches together with development and application advances of the molecular protein engineering are reviewed. The site-directed mutagenesis and chemical modification are discussed. The advantages of site-specific mutagenesis over chemical modification are its high specificity of site mutation and almost free approach of engineering proteins by fusing new and foreign functional groups or deleting some original groups.