摘要
通过对木霉的孢悬液进行紫外线和亚硝酸钠复合诱变,经刚果红培养基鉴定筛得5株突变菌株。利用液体培养发酵,对各菌株的CMC酶活测定发现,其中Ⅱ号、Ⅲ号突变菌株的CMC酶活分别比原菌株提高了150%和65%,并均具有很好的抗葡萄糖阻遏效应。
By means of treating Tr..4ureoviride spore with ultraviolet radiation and NaNO2, after selecting at Congo red medium, the five induced mutant strain were obtained. Determining CMC enzyme activity of every strain cultivated at liquid medium, the result showed that CMC enzyme activity of Ⅱ strain and Ⅲ strain were increased 150% and 65% than the former strain, and they were all resistance to glucose.
出处
《黑龙江八一农垦大学学报》
2005年第4期13-16,共4页
journal of heilongjiang bayi agricultural university
关键词
木霉
复合诱变
葡萄糖阻遏效应
Trichoderma aureoviride
compound induced mutant
resistance to glucose
