期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

薄层扫描法测定生物转化中多羟基甾体化合物 被引量：1

Determination of Polyhydroxysteroids in Biotransformation by TLC-Densitometry
下载PDF
导出
摘要 建立了生物转化中多羟基甾体的薄层扫描测定法。以硅胶GF254为吸附剂,氯仿-甲醇(8∶1)混合液为展开剂,测定波长为350nm,参比波长235nm,可测定生物转化中多羟基甾体的含量。该方法准确、简便、回收率好。 TLC-densitometry of polyhydroxysteroids in biotransformation of steroids was established. Silica gel GF254 was selected as absorbent, and the mixture of CHCI3 and MeOH （8：1） as developing solvent. The determination wavelength and reference wavelength were 350nm and 235 nm respectively. This convenient method could be used in the determination of polyhydroxysteroids in biotransformation of steroids.
作者 葛文中 李和平 朱玉 刘宏民
机构地区 黑龙江八一农垦大学生命科技学院 郑州大学化学系
出处 《黑龙江八一农垦大学学报》 2005年第4期90-92,共3页 journal of heilongjiang bayi agricultural university
基金 黑龙江省教育厅科研项目资助(10551221)。
关键词 薄层扫描法 多羟基甾体 生物转化 含量测定 TLC-densitometry polyhydroxysteroids biotransformation quantitative analysis
分类号 TQ467.8 [化学工程—制药化工]
  • 相关文献

参考文献3

  • 1Finamore E.,Minale L.,Riccio, R. et al. Novel Marine Polyhydroxylated Steroids from the Starfish Myxoderm platyacantum[J].J Org. Chem, 1991, (56):1146-1153.
  • 2Riccio R, Minale L, Iorizzi M, et al. Starfish saponins. Part 34. Novel Steroidal glycoside sulphates from the starfish Asterias amurensis[J].J. Chem. Soc. Perkin Trans 1,1988, (6):1337-1347.
  • 3Joannou GE. Identification of 15 β -hydroxylated C21 steroids in the neonatal period: The role of 3 α,15β,17α-trihydroxy-5β-pregnan-20-one in the perinatal diagnosis of congenital adrenal hyperplasia (CAH) due to a 21-hydroxylase deficiency[J].J Steroid Biochem, 1981, (14):901-912.

同被引文献7

  • 1Shao-Min Wang, Wen-Zhong Ge, Hong Min Liu. Syntheses of acetylated steroid glycosides and selective cleavage of O-acetyl groups in sugar moiety[J].Steroids, 2004, (69) : 599-604.
  • 2Muhammad I C, Iffat B, Syed AS, et al. Microbial Hydroxylation of pregnenolone derivatives[J].Chem.Pharm. Bull., 2005, 53 (11) : 1455-1459.
  • 3Maria JC, Marta S M, Alejandro JR, et al. Evaluation of the antiviral activity of natural sulfated polyhydroxysteroids and their synthetic derivatives and analogs[J].Steroids, 1999, (64) : 335-340.
  • 4Fernandes P, Cruz A, Angelova B, et al. Microbial conversion of steroid compounds: recent developments[J].Enzyme and Microbial Technology, 2003, (32) : 688-705.
  • 5Shashi B, Mahato F, Subhadra G. Advances in,microbial steroid biotransformation[J].Steroids, 1997, (62) : 332-345.
  • 6Hu S-H, Gilles G, Robert A. Microbial transformation of steroids: contribution to14α -hydroxylations[J].Steroids, 1995, (60) : 337-352.
  • 7Perry EK. The cholinergic hypothesis-ten years on[J].Br. Med. Bull., 1986, (42) : 63-69.

引证文献1

黑龙江八一农垦大学学报
2005年 第4期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部