杏龙平喘方对哮喘小鼠模型白细胞介素-5及氧自由基代谢产物的影响

Effect of Xinglong Pingchuan recipe on interleukin-5 and the inflammagen related to oxygen free radical in asthmatic mice

目的:观察杏龙平喘方对哮喘小鼠白细胞介素-5(IL-5)和氧自由基物质的影响,探讨其作用机制。方法:采用卵白蛋白(OVA)腹腔注射致敏和雾化激发的方法建立小鼠哮喘模型,然后用杏龙平喘方对哮喘小鼠进行干预。干预完成后,对外周血白细胞和嗜酸性粒细胞进行计数,采用ELISA法检测小鼠血清及肺组织中IL-5水平,分光光度法检测小鼠肺组织中超氧化物歧化酶(SOD)、谷光甘肽过氧化物酶(GPx)及丙二醛(MDA)含量。结果:杏龙平喘方干预组小鼠与哮喘模型组小鼠相比,前者外周血中嗜酸性粒细胞数目下降(P<0.01);前者肺组织及血清中IL-5浓度明显低于后者(均P<0.01);前者肺组织中SOD和GPx活性增高,SOD活力在2组间差异有统计学意义(P<0.01);且前者MDA含量明显下降(P<0.05)。结论:降低哮喘小鼠肺组织和血清中IL-5浓度,提高肺组织抗氧化能力,可能是杏龙平喘方治疗哮喘的作用机制之一。

Objective To observe the effect of Xinglong Pingchuan recipe （XLPCR）on interleukin-5 （IL-5）, superoxide dismutase （SOD）, glutathione peroxidase （GPx）, and malondialdehyde （MDA） in mouse asthma models, and to explore its mechanism in treating asthma. Methods The mouse asthma models were established by sensitization and challenge with ovalbumin （OVA）. The asthma model was treated with XLPCR. At last, the number of white blood cells and eosinophil was counted, and the concentrations of inflammation factors such as IL-5, SOD, GPx, and MDA in the serum or the lung tissue of each mouse were detected. Results Compared with the asthmatic group, the number of eosinophil in the XLPCR group decreased significantly （P〈0.01） ; the concentration of IL-5 in the XLPCR group significantly decreased in the serum or the lung tissue （all P〈0.01） ; and the concentrations of SOD and GPx in the XLPCR group increased （P〈0.01 and P〉0.05, respectively）. On the other hand, the concentration of MDA in the XLPCR group was significantly lower than that of the asthmatic group （P〈0.05）. Conclusion XLPCR might inhibit the airway inflammation by decreasing the IL-5 level and adjusting the balance of oxidants/antioxidants.