L-精氨酸与小剂量阿司匹林共同使用增强抑制血小板聚集功效以及对胃粘膜保护作用的研究

Effects of Co-administration of L-arginine and ASA on platelet aggregation and gastric damage

目的评价L精氨酸(LArg)与小剂量阿司匹林(ASA)共同使用是否能够增强抑制血小板聚集的作用以及对胃粘膜的保护效果。方法体外实验用枸橼酸抗凝法提取新西兰大白兔富血小板血浆(PRP),用透光度法测定血小板聚集。体内实验使用Wistar大鼠和昆明小鼠,用水浸实验以及注射利血平制作动物胃溃疡模型。给予LArg和ASA后7d后处死动物,观察胃粘膜损伤程度。结果3mmol·L-1LArg与小剂量阿司匹林同时使用时,抑制血小板聚集的作用明显增强,血小板聚集率为6.6%。同样浓度的LArg单独使用时,血小板聚集率为93.9%。二者之间存在明显差异。对胃粘膜保护作用的研究,水浸和利血平两种动物模型均显示LArg给药明显抑制了胃粘膜损伤,对胃粘膜的保护效果与抗溃疡药物法莫替丁几乎相同。结论LArg与小剂量ASA联合使用能够增强抗血小板聚集的效果,同时能够对抗ASA胃粘膜损伤的副作用。

Aim To investigate the effects of co-administration of L-Arginine （L-Arg） and aspirin （ASA） on platelet aggregation in vitro and gastric damage in vivo. Methods Citrate anti-coagulation venous blood was obtained from rabbits. The blood was centrifuged at 1 100 r· min^-1 for 10 min to obtain platelet-rich plasma （PRP）. 500 μl of PRP was poured into a cuvette, and then incubated with various concentrations of L- Arg and a small dose of ASA for 5 min. Platelet aggregation was assayed with Chrono-Log platelet aggregometer by changes in light transmission of platelet suspensions. After co-administration of L-Arg and ASA for 7days to rats, gastric damages were induced by water immersion restraint stress or reserpine. Results Coadministrateion of L-Arg and a small dose of ASA strengthened the inhibitory effects on platelet aggregation. Platelet aggregation rate was 6. 6% using 3 mmol·L^-1 of L-Arg and small dose of ASA. Platelet aggregation rate was 93.9% using the same concentration of L-Argalone. 1 g· kg^-1 of L-Arg and 0.1 g· kg^-1of ASA co-administration for 7 days,significantly reduced gastric damage induced by water immersion restraint stress on Wistar rat. Similar results were observed on other animal experiments with gastric lesions induced by reserpine. The effects of L-Arg on prevention of gastric lesion were almost same as that of famotidine.Conclusion Co-administration of L-Arg and ASA can enhance the inhibitory effects on platelet aggregation and prevent gastric damage.