壳聚糖复合不对称膜的制备及生物组织相容性研究

Preparation and histocompatibility analysis of composite asymmetric chitosan membrane modified by blending with carboxymethyl-chitosan

用分步流延干燥法制备了一种由壳聚糖(CS)、聚乙烯醇(PVA)、羧甲基壳聚糖(CM-CS)构成的新型人工皮肤医用材料--壳聚糖复合不对称膜(C-P-C膜),其上层为CS层,中间为PVA层,下层为CM-CS层.研究结果表明,可通过调节CS、PVA、CM-CS的加入量来控制复合不对称膜各层的厚度和膜的水蒸气通透性,C-P-C膜具有良好的透光性和吸水率.生物相容性实验发现,C-P-C膜是无毒材料,不会引起创伤感染,随着植入C-P-C膜时间的延长,组织炎症反应明显减轻,6周时,炎症浸润程度低于手术缝合线.C-P-C膜能够明显地促进创面愈合,能有效地密封出血创面且具有显著的止血作用.

A novel composite asymmetric chitosan membrane（C-P-C） was prepared with casting method and evaluated as an artificial skin iatric-material. In this membrane, chitosan （CS） was the top-layer and carboxymethyl-chitosan （CM-CS） was the sub-layer separated by polyvinyl alcohol （PVA） layer. C-P-C membrane can control water vapor evaporation, and has excellent light transmittance and fluid drainage ability. The tissue-compatible experiment indicated that C-P-C membrane does not possess antigenicity and toxicity, and does not infect a wound with disease germs. The C-P-C membrane is hemostatic and the wound covered with it can be healed quickly. The results show that C-P-C membrane has excellent histocompatibility.