维A酸对HL-60细胞增殖与NF-κB、VEGF表达的影响

Effect of Tretinoin on Proliferation of HL-60 Cells and Expressions of NF-κB and VEGF

目的:观察维A酸对HL-60细胞增殖与NF-κB、VEGF表达的影响,以探讨维A酸治疗白血病的作用机制。方法:将不同浓度的维A酸作用于HL-60细胞72h后,采用MTT法观察细胞生长,用ELISA法检测上清液中VEGF的含量,并用流式细胞术检测NF-κB活性表达及细胞周期。结果:HL-60细胞经维A酸作用72h后生长显著受抑制,实验组HL-60细胞上清液中VEGF含量、NF-κB表达下降,10-6M、10-7M组与对照组相比差异显著(P<0·05),而10-8M组与对照组比较,无显著差异(P>0·05),其中10-6M组NF-κB表达与VEGF含量呈显著正相关(r=0·55,P<0·01,n=6),各治疗组G1期细胞明显升高,10-6M组、10-7M组与对照组相比,差异显著(P<0·01),而10-8M组与对照组比较,无显著差异(P>0·05)。结论:维A酸对HL-60细胞增殖有抑制作用,其作用机制可能与NF-κB活性及VEGF表达水平下调有关。

Objective：To observe effect of Tretinoin on proliferation of HL-60 cells and on expressions of NF-kB and VEGF in order to explore the mechanism of Tretinoin in treatment of leukemia. Methods：HL-60 cells were cultured in RPMI 1 640 medium with Tretinoin in the final concentrations of 10^-8mol/L, 10^-7 mol/L and 10^-6 mol/L,respectively. After the treatment of the cells for 72 h,the expression of NF-kB and cells cycle were examined with flow cytometry （FCM） ;VEGF expression was detected with enzyme linked immunosorbent assay （ELISA） ; MTT method was used to observe the cells proliferation. Results：Proliferation of HL-60 cells were significancly inhibited 72 hours after treatment with Tretinoin;the expression of NF-kB and VEGF in 10^-7 mol/L and 10^-6 mol/L groups were significance decreased than in control group （P 〈 0.05 ） , and their expressions in 10^-8mol/L were not significantly different from control group （P 〉 0. 05 ）. In 10^-6 mol/L group, the expression of NF-kB was significantly correlated positively with concentration of VEGF （ r = 0. 55, P 〈 0. 01 , n = 6 ）. The proportion of cells in G; phase in 10^-6 mol/L and 10^-7 mol/L groups was significantly increased than in control group （P 〈 0.01 ） ;that in 10^-8 mol/L groups was not greatly different from control group（P 〉 0.05 ）. Conclusions：Tretinoin has inhibition effect on proliferation of HL-60 cells and the mechanism of this inhibition might be related with down-regulated expressions of NF-kB and VEGF.