HPLC法测定雾灵鼻通中盐酸麻黄碱和地塞米松磷酸钠的含量

Determination the Content of Ephedrine Hydrochloride and Dexamethasone Sodium Phosphate in Wuling-bitong by HPLC

目的:建立HPLC法测定雾灵鼻通中盐酸麻黄碱和地塞米松磷酸钠的含量。方法:采用shim-pack CLC—ODS(6 mm ×150 mm)色谱柱,以甲醇:0．34％磷酸二氢钾(55:45)为流动相,流速1 ml·min-1,检测波长257 nm。结果:盐酸麻黄碱和地塞米松磷酸钠的线性范围分别为0．1～0．5 mg·ml-1和0．01～0．05 mg·ml-1;r均为0．999 9;样品的平均回收率分剐为100．1％ (RSD=0．8％,n=5)和100．1％(RSD=1．2％,n=5)。结论:本方法快速、简便、结果准确,可用于控制该制剂的质量。

Objective： To establish a HPLC method for determination the content of ephedrine hydrochloride and dexamethasone sodium phosphate in Wulingbitong. Method： The Shim-pack CLC-ODS column （6 mm × 150 mm, 5 μm） was used. The mobile phase was methanol-0.34% potassium dihydrogen phosphate solution （55：45 ）. The flow rate was 1 ml·min^-1. The detection wavelength was 257 nm. Result： The linear ranges of ephedrine hydrochloride and dexamethasone sodium phosphate were at 0.1-0.5 mg. ml^-1 and 0.01-0.05 mg.ml^-1 respectively. They both showed a good linear correlation. The average recoveries was 101.1% （RSD = 0.8% ） and 101.1% （ RSD = 1.2% ）. Conclusion： This method is rapid and accurate. It can be used for quality control of the preparation.