黑大豆种皮花色苷的提取及其抗氧化作用研究

Extraction and antioxidation of anthocyanin of black soybean seed coat

以黑大豆皮为材料,采用二次回归正交旋转组合设计对其花色苷的提取工艺进行了优化研究。结果表明,最佳提取参数为温度60℃、时间1h、乙醇浓度60%、料液比1∶40。对黑大豆种皮花色苷含量与总抗氧化能力之间的相关性分析表明,二者之间存在极显著的线性关系(P<0.01),且黑大豆种皮花色苷提取物表现出较强的清除OH·、O-2·及有机自由基DPPH·的体外抗氧化作用,其清除能力是维生素C的1.6倍、2.2倍、1.4倍。

The technology for extracting anthocyanins of black soybean seed coat was optimized by quadric regression orthogonal rotary tests, The results showed that the optimum extraction parameters were as follows： temperature 60℃, time 1 h, ethanol concentration 60%, the ratio of black soybean seed coat mass to ethanol volume 1 ： 40. It also showed that the most significant （P 〈 0. 01） correlations existed between total antioxidant capacity and anthocyanins contents. Moreover, the scavenging free radical capacity of anthocyanins in black soybean seed coat to OH· , O2^-· and DPPH · was 1. 6 times, 2. 2 times and 1. 4 times as large as that of Vitamin C, respectively.