半边旗提取物5F影响高转移卵巢癌细胞HO-8910PM细胞周期的实验研究

Study on the Effect and Its Mechanisms of 5F from Pteri semipinnata L. on the Cell Cycle of Highly Metastatic Ovarian Carcinoma HO-8910PM Cells

目的探讨半边旗提取物5F对人高转移卵巢癌细胞HO8910PM细胞周期的影响及其作用机制。方法以MTT法检测5F对高转移卵巢癌细胞HO8910PM细胞增殖的影响;流式细胞术分析5F对HO8910PM细胞周期的影响;Westernblot法分析NFκB(P65)、FAK的表达及FAK酪氨酸磷酸化水平。结果不同浓度的5F分别处理细胞24h后,对HO8910PM细胞增殖有明显的抑制作用;流式细胞术分析表明5F使G0/G1期的细胞减少,阻断细胞于G2/M期;同时,5F可使NFκB(P65)的表达下调,上调FAK的表达,并降低FAK酪氨酸磷酸化水平。结论5F对HO8910PM细胞周期的影响与NFκB(P65)表达下调及FAK的表达上调有关。

Objective：To study the effect and its possible mechanism of 5F from Pteri semipinnata L on the cell cycle of human highly metastatic ovarian carcinoma HO-8910PM cells. Methnds： MTT assay was used to examine the effect of 5F on proliferation of HO- 8910PM cells after 24 hours treatment;The cell cyele was assessed by flowcytometry （FCM） ;The expression level of NF-KB（ p65 ） , FAK and the level of phosphorylated FAK were assessed by Western blot analysis. Results ：5F from Pteris semipinnata L. could inhibit the proliferation of HO-8910PM cell and block the cell cyele at G2/M phase. The expression level of NF-KB（ P65 ） protein decreased obviously in HO-8910PM cells treated with 25 - 100 μmol/L 5F for 24 hours,and the effect appeared in a dose-dependent manner. 5F up-regulated significantly the expression of FAK,down-regulated the level of phosphorylated FAK. Conclusion ：The effect on cell cycle of 5F from Pteri semipinnata L is involved in the expression of NF-KF（ p65 ） ,FAK and the level of phosphorylated FAK.