严重烧伤休克大鼠淋巴微循环、细胞因子及淋巴管内皮细胞超微结构的变化

The Dynamic Changes of Lymphatic Motion and Endothelium Ultrastructure of Burn Shock Rats

目的研究严重烫伤休克期大鼠淋巴微循环及淋巴液中肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、白细胞介素-8(IL-8)水平的变化,观察肠系膜淋巴管内皮细胞超微结构变化。方法将Wistar大鼠随机分为A烧伤补液组、B烧伤非补液组和C对照组。在A组和B组动物背部造成30%TBSAⅢ度烫伤,A组补液抗休克,B、C组不予补液。观察大鼠肠系膜淋巴管伤后6、24、48h的运动变化,计算淋巴管收缩频率,检测淋巴液TNF-α、IL-6、IL-8水平的变化。利用扫描电镜观察肠系膜淋巴管内皮细胞超微结构变化。结果烧伤休克淋巴管收缩频率降低,电镜下见肠系膜淋巴管内皮细胞肿胀,内皮细胞连接破坏。淋巴液中TNF-α、IL-6、IL-8的水平升高。与A组比较,B组收缩频率显著降低,淋巴液中TNF-α、IL-6、IL-8的水平显著升高。结论严重烫伤休克大鼠淋巴管扩张,运动减少,肠系膜淋巴管内皮细胞损伤,淋巴液中TNF-α、IL-6、IL-8的水平升高。液体复苏能够改善淋巴循环。

Objective To study the dynamic changes of lymphatic motion and ultrastructural changes of lymphatic endothelium, and measure tumor necrosis factor-α（TNF-α）, interleukin-6（IL-6） , interleukin-8 （IL-8） level in lymph of burn shock rats . Method 42 male adult Wistar rats were randomly divided into the burn resuscitation group （group A, n = 18）, the burn non - resuscitation group （group B. n = 18） and the control （ group C, n = 6）. Using inverted microscopy and digital image processing to observe the motive frequency of the rat mesenteric lymphatic vessels and measure the concentrations of TNF-α, IL-6, IL-8 in lymph at 6, 24, 48 hours post burn. Results In group A and B, the contractile frequency of the mesenteric lymphatic vessels was decreased, together with ultrastructural disruptions such as swelling and contacts disruption of lymphatic endothelium, despite the increasing level of TNF-α, IL-6, IL-8 in lymph compared with group C. The contractile frequency and the level of TNF-α, IL-6, IL-8 in group A were significenfly different in group B. Conclusion In the burn shock stage, the motion of the lymphatic vessels was decreased together with ultrastructural disruptions in lymphatic endothelium, and the level of TNF-α, IL-6, IL-8 in lymph was increased. Fluid resuscitation could improve the lymph circulation.