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RT-PCR法检测大鼠组织中GLUT8基因表达特异性 被引量：1

Specificity of GLUT8 Gene Expression in Rat Testis Detected by RT-PCR
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摘要 采用半定量RT_PCR技术检测青春前期和成年大鼠的主要组织中GLUT8基因mRNA表达水平.实验结果显示,葡萄糖转运蛋白GLUT8基因在成年和青春前期大鼠的睾丸组织中都有高度表达,在心脏和肾脏组织中有少量表达,在肝、脾等组织中的表达是微量的;在成年大鼠睾丸组织中,Leydig细胞、睾丸生精细胞以及附睾的精子细胞中GLUT8基因都有大量的表达,其中以在Leydig细胞中的表达最丰富,而且GLUT8基因在睾丸生精细胞中的表达水平高于附睾的精子细胞.结果说明GLUT8蛋白主要分布在大鼠睾丸组织中,并且主要在Leydig细胞和精子细胞中参与葡萄糖转运和能量代谢与供应. Tissue-specific expression of GLUT8 gene in rat tissues was investigated. GLUT8 gene expression was detected by RT- PCR method. The results revealed that GLUT8 is expressed predominantly in the testis,in smaller amounts in heart and kidney,and in negligible amounts in liver and spleen. Furthermore, GLUT 8 mRNA was found to be highly expressed in Leydig cells and testicular and epididymal germ cells. The data of RT - PCR also indicated that, in prepubertal rat（20day-old）tissues, GLUT 8 expression was comparatively much lower than that in the adult rat tissues. These results indicated that GLUT 8 is predominantly distributed in Leydig cells and sperm cells.
作者 陈粤
机构地区 汕头大学理学院生物学系
出处 《汕头大学学报（自然科学版）》 2005年第3期60-64,74,共6页 Journal of Shantou University：Natural Science Edition
关键词 RT-PCR GLUT8基因 基因表达 大鼠组织 RT-PCR GLUT8 gene gene expression rat tissue
分类号 Q954 [生物学—动物学]
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参考文献10

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同被引文献29

  • 1唐万欣,4黄颂敏,柳飞,沙朝晖.高糖和胰岛素对肾小球系膜细胞葡萄糖转运蛋白4mRNA表达及细胞骨架纤维状肌动蛋白的影响[J].中华肾脏病杂志,2004,20(5):351-354. 被引量：10
  • 2罗萍,许钟镐,孙广东,张冬梅,苗里宁,李才.ERK1/2对高糖刺激肾小球系膜细胞葡萄糖转运蛋白1和p27^(Kip1)表达的作用[J].中华肾脏病杂志,2006,22(9):569-571. 被引量：7
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  • 5Kumagai A K.Glucose transport in brain and retina:implication in the management and complication of diabetes[J].Diabetes Metab Res Rev,2006,15(41):261-273.
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  • 7Fischereder M,Schroppel B,Wiese P,et al.Regulation of glucose transporters in human peritoneal mesothelial cells[J].J Nephrol,2003;16(1):103-109.
  • 8Galardo MN,Riera M F,PellizzariE H,et al.Regulation of expression of Sertoli cell glueose transporters 1 and 3 by FSH,IL1 beta,and bFGF at two different time-points in pubertal development[J].Cell Tissue Res,2008,334(2):295-304.
  • 9Chia-Hao Chen,Hao-Jui Hsu,Yun-Ju Huang,et al.Interaction of flavonoids and intestinal facilitated glucose transporters[J].Planta Med,2007,73:348-354.
  • 10Meller AM,Jensen NM,Pildal J,et al.Studies of genetic variability of the glucose transporter2 promoter in patients with type 2 diabetes mellitus[J].J Clin Edocrinol Metab,2001,86:2181-2186.

引证文献1

汕头大学学报（自然科学版）
2005年 第3期

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