日本血吸虫谷胱甘肽S－转移酶小鼠免疫试验

THE MICE VACCINATION EXPERIMENT WITH GLUTATHIONE S-TRANSFERASE FROM SCHISTOSOMAJAPONICUM

用谷胱甘肽琼脂糖亲和层析柱纯化中国大陆株日本血吸虫成虫谷胱甘肽Ｓ－转移酶（ＧＳＴ）。把纯化的ＧＳＴ结合福氏性剂免疫了二批达ＢＡＬＢ／ｃ小鼠，获得了２９．５８～３２．７１％的减虫率和５２．９４～６８．１３％的粪便减卵率。ＥＬＩＳＡ试验表明免疫小鼠产生了体液免疫应答，免疫印迹试验（ＷｅｓｔｅｒｎＢｌｏｔｔｉｎｇ）显示日本血吸虫水溶性成虫抗原和ＧＳＴ抗原的２６、２８ｋＤ蛋白被免疫小鼠血清所识别。

The Glutathione S-Transferase (GST) was Purified by glutathione-agarose affinity chromatography column from the Chinese strain of Schistosoma japonicum, and the purifiedGST antigen was used to vaccinate BALB/c mice combined with Freund adjuvant.29.58% to32.71% worm reduction and 52.94% to 68.13 % faeces egg reduction were obtained from twoexperiments. The result of ELISA had detected the specific humoral immunoreaction in the bodies of immunized mice.Western Blotting had shown that the molecule of 26 KD and 28 KDof S.japonicum soluble adult worm antigen(SWAP)or purified GST antigen could be recognized by mice serum immunized with GST.