霍乱疫苗效力试验替代方法的研究

A primary study of substitute for efficacity test of Cholera vaccine

目的研究霍乱疫苗效力试验的替代方法。方法以不同剂量霍乱疫苗免疫小鼠,用间接ELISA方法检测小鼠全血的抗菌、抗毒抗体滴度,小鼠毒菌攻击法计算保护率。结果抗毒、抗菌抗体水平与免疫剂量和攻毒后的动物保护率成正相关,结果稳定。结论可以通过检测高免疫剂量组实验动物的抗菌、抗毒抗体替代以往小鼠攻毒法的效力试验。

Objective To find the relationship oi the antiserum uter, protection ratio and immunization dose of the cholera vaccine, and to develop an appropriate efficacity test. Methods The mice were immunized by different doses of Cholera vaccine. Titer of antiserum against cholera strain and toxin were tested by indirect enzyme-linked immunosorbent assay （ELISA）. protection ratio were also calculated. Results Antiserum tilter, immune dose and animal protection ratio showed the relationship of direct proportion. Conclusion A new method has been developed to substitute for the traditional efficacity test.