时辰对普鲁卡因、丁卡因和布比卡因致惊厥作用半数有效量的影响

Effect of chrono on median effective dose of convulsion induced by procaine,tetracaine and bupivacaine

目的观察时辰对普鲁卡因、丁卡因和布比卡因惊厥半数有效量(ED50)的影响。方法180只小鼠随机分3组即普鲁卡因组、丁卡因组、布比卡因组,按不同时辰每组再随机分为4个亚组。分别于2∶00时、8∶00时、14∶00时和20∶00时腹腔注射普鲁卡因、丁卡因和布比卡因,以小鼠惊厥为观察指标,用序贯法测定各时间点的ED50。结果普鲁卡因在8∶00时的惊厥ED50明显低于2∶00时、14∶00时和20∶00时的ED50(P<0.05),其余各时辰之间的ED50比较,无显著性差异(P>0.05);丁卡因和布比卡因在14∶00的惊厥ED50均明显低于2∶00时、8∶00时和20∶00时的ED50(P<0.05),其余各时辰之间的ED50比较,无显著性差异(P>0.05)。结论普鲁卡因、丁卡因和布比卡因所致惊厥ED50作用具有时辰依赖性。

Objective To observe the effects of chrono on the median effective dose（ED50） of convulsion induced by anaesthetics （ pricaine , tetracaine and bupivacaine ）. Methods One hundred and eighty mouse were randomly divided into 3 groups which were then divided into four subgroups. Each subgroup had 15 mouse. Procaine, tetracaine and bupivacaine were intraperitoneally injected at 2：00,8：00, 14：00 and 20：00, respectivdy. The convulsion was investigated and ED50 at different times was determined by up-down .sequential allocation in mouse. Results The convulsive ED50 of procaine at 8 ： 00 was significantly lower than that at 2：00,14：00 and 20：00（ P 〈 0.05）. The convulsive ED50s of tetracaine and bupivacaine at 14 ： 00 were significantly lower than that at 2 ： 00,8 ： 00 and 20 ： 00 （ P 〈 0.05 ）. Conclusion The effects of convulsive ED50s of procaine, tetracaine and bupivacaine were chrono-dependent.