新生仔猪小肠生长发育及肠黏膜部分基因的表达

Small intestinal development and expressions of some genes in the small intestinal mucosa of neonatal pigs

随机选择10头初生仔猪,均分为新生组(D0)和自然哺乳3d组(D3)。结果表明,D3组仔猪小肠长度和质量、黏膜质量、黏膜DNA、RNA和蛋白质含量以及乳糖酶、麦芽糖酶和L亮氨酸氨肽酶(LAP)活性高于D0组(P<0.05或P<0.01),D3组小肠黏膜IGF1及其受体以及LAP基因表达水平高于D0组(P<0.05)。结果提示:新生仔猪肠道的迅速发育与黏膜IGF1受体和IGF1基因的上调有关,LAP活性增加是该基因表达水平提高的结果。

To examine the mechanism of the rapid development of gastrointestinal tract in neonatal pigs, ten newborn piglets were selected and divided into group D0 （ n = 5） and D3 （ n = 5）. The piglets of Do were slaughtered after birth and those of D3 were reared by sows for three days. The results showed that the length and the weight of small intestine, the contents of DNA, RNA and protein, the activities of lactase, maltase and leucine aminopeptidase （LAP） in the small intestinal mucosa of D3 were higher （P〈0. 05 or P 〈0.01） than that of D0. In the intestinal mueosa, expressions of LAP, IGF-1 and IGF-1 receptor in D3 were higher than that of Do. These results suggest that the fast development of gastrointestinal tract in neonatal pigs is related to the up-regulation of IGF-1 receptor and IGF-1 expression in the small intestinal mucosa. In addition, the increase of LAP activity is partially the result of up-regulation of LAP gene expression.