萘乙酸抑制K562细胞增殖作用的初步研究

Primary study of the inhibitive effect of naphthalene acetic acid on K562 cell proliferation

目的研究萘乙酸(NAA)对K562细胞增殖的抑制作用。方法应用MTT法、细胞计数法、流式细胞术、分裂指数分析、tunnel法以及姊妹染色单体交换(sister chromatid exchange,SCE)和微核制备,对NAA处理的K562细胞进行细胞增殖、细胞周期、凋亡以及染色体损伤检测。结果与对照组相比,NAA可以有效抑制K562细胞的生长,并将细胞阻滞在G1期,降低细胞分裂指数,促进细胞凋亡,提高K562细胞SCE频率以及微核形成率。结论NAA具有抑制K562细胞增殖的作用。

Objective To explore the effect of naphthalene acetic acid（NAA） on animal cells. Methods K562 cells were treated in vitro with different doses of NAA （0, 50 and 100 μmol/L）. The cell proliferation, cell cycle, cell division index and apoptosis was observed by means of MTT, cell enumeration, and flow cytometry. The sister chromatid exchange （SCE） and micronucleus were tested. Results The proliferation of K562 cell was inhibited by NAA compared with that of the controls; the cell cycle was arrested at G1, and the cell division index reduced; apoptosis cells increased lightly in NAA experiments; the frequency of SCE and micronuclear formation were increased by NAA treatment. Conclusion NAA has antiproliferative activities on K562 cell.