摘要
目的探讨鼠重组成骨蛋白1(rOP1)在大鼠肾脏缺血再灌注损伤过程中对氧自由基、细胞凋亡的影响及其对肾脏的保护作用。方法观察静脉应用rOP1250μg/kg体重,大鼠肾脏缺血60min,再灌注24h后,血浆超氧化物歧化酶(SOD)、脂质过氧化物丙二醇(MDA)、内皮素1(ET1)的变化,及其对肾脏细胞凋亡的影响。结果rOP1组和缺血再灌注组血浆SOD分别是(108±20)和(86±11)KNU/L,MDA分别是(8.90±0.49)和(11.70±0.81)μmol/L,ET1分别是(130±39)和(179±45)ng/L,rOP1组SOD水平显著性高于缺血再灌注组(P<0.05),而MDA和ET1水平显著性低于缺血再灌注组(P<0.05)。两组肾脏细胞凋亡指数分别是(5.72±3.08)%和(8.85±3.52)%,rOP1组显著性低于缺血再灌注组(P<0.01)。上述两组肾脏的的Miller’s评分分别是(2.0±0.3)和(3.4±0.5),rOP1组显著低于缺血再灌注组(P<0.01)。结论rOP1减轻氧自由基对肾脏缺血再灌注损伤,降低肾脏细胞凋亡指数,对肾脏缺血再灌注损伤具有保护作用。
Objective To investigate the influence of rOP-1 on oxygen-delived free radicals and renal cell apoptosis in rat kidneys with .ischemia-reperfusion injury and it's protective effect on injured kidneys. Methods The changes of plasma superoxide dismutase (SOD), malondialdehyde (MDA) and endothelin-1 (ET-1) as well as the influence of 250μg·kg-1 of rOP-1 applied intravenously on renal cell apoptosis were observed 24 hours after reperfusion in rat kidneys of ischemia for 60 minutes. Results The levels of plasma SOD, MDA and ET-1 in rOP-1 group were ( 108±20) KNU/L, (8.90±0.49)μmol/L and (130±39 ) ng/L, respectively and those in ischemia-reperfusion group were (86±11 ) KNU/L, ( 11.70±0.81 ) /μmol/L and ( 179±45 ) ng/L, respectively. The level of SOD in rOP-1 group was significantly higher than that in ischemia-repeffusion group (P〈0.05), but the levels of plasma MDA and ET-1 were significantly lower than those in ischemia-reperfiasion group (P〈0.05). The indexes of renal cell apoptosis in the two groups were (5.72±3.08) % and (8.85±3.52) %, respectively and index of renal cell apoptosis in rOP-1 group was significantly decreased than that of ischemia-reperfusion group ( P〈0.01). The Miller's scorings of both two group mentioned above were (2.0±0.3) and (3.4±0.5) and the scoring of rOP-1 group was significantly lower than that of is chemia-reperfusion group (P〈0.01). Conclusion rOP-1 diminishs renal ischemia-reperfusion injury induced by oxygen-delived free radicals, decreases index of renal cell apoptosis and possesses protective effect on kidney with ischemia- reperfusion injury.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2005年第9期1046-1047,共2页
Chinese Journal of Experimental Surgery