甲壳素短纤维对聚己内酯体内的降解作用

Degradation performance of chitin short fiber reinforced polycaprolactone composite in vivo

目的对纯聚己内酯(PCL)和甲壳素短纤维增强PCL复合材料进行体内降解实验研究,观察其理化性能变化及组织反应情况,为临床应用提供有价值的实验依据。方法将纯PCL和甲壳素短纤维增强PCL植入兔背部脊柱两侧肌肉内,于2、4、8、12、16、24周取材,分别测试两种材料的生物吸收率、弯曲强度和弯曲模量,并行组织学和透视电镜观察。结果甲壳素短纤维增强PCL初始强度大于纯PCL,在体内植入过程中,生物吸收率大于纯PCL,力学性能先高后低,强度和模量值24周时与初始值相差不大。未见材料周围组织变性、坏死或肉芽肿异常增生现象。结论PCL复合甲壳素纤维后,加快降解速度,提高力学性能,材料组织界面炎性反应轻,是一种具有开发价值的新型胸壁缺损修补及骨科内固定材料。

Objective To investigate the degradation properties of pure polycaprolactone （PCL） and chitin short fiber reinforced PCL composite in vivo in order to provide useful scientific basis for clinical application. Methods Pure PCL and chitin short fiber reinforced PCL were implanted in the dorsal muscle of rabbits for periods of 2, 4, 8, 12, 16, 24 weeks. Then mass weight loss measurement, mechanical properties assessment, histological and SEM observation were performed to study the change of the tested materials in the process of degradation. Results The initial strength of chitin short fiber reinforced PCL was much higher than that of pure PCL. In the process of degradation of chitin short fiber reinforced PCL, the rate of weight loss was faster than pure PCL. Strength and Modulus of chitin short fiber reinforced PCL had no significant change in 24 weeks as compared with initial ones. Conclusion The composites have much excellent properties and may become novel biomaterials for clinical use of reconstruction of chest wall defects and internal fixation of bone fracture.