一种改进大鼠肺移植模型的建立

Experimental study on the improved model of rat lung transplantation

目的改进大鼠原位单肺移植实验方法,建立稳定的动物模型。方法取近交雄性SD大鼠,行原位灌洗和供体心肺联合切取,应用cuff技术吻合肺动、静脉,prolene线连续吻合支气管。术后通过胸部X线和动脉血气分析监测移植肺存活情况。结果共完成26例大鼠肺移植手术,成功23例,失败3例,手术成功率为88.5%,术后7d存活率为100%。手术操作简便,技术稳定。结论该模型的改进为肺移植及其免疫学等方面的研究奠定了良好的基础。

Objective To improve the technique of rat single lung transplantation and establish a stable animal model. Methods The SD rats served as donor and recipients. In the donor operation, the heart-lung block was flushed in situ and harvested. The left pulmonary artery and vein were anastomosed using cuff technique. Left main bronchus was reconstructed with continuous suture of prolene. The graft function was monitored by thoracic X ray and arterial blood gas analysis. Results In 26 cases of single lung transplantation in rats, the operation was successfully carried out in 23 cases and failed in 3 cases with the successful rate being 88.5 %. The survival rate was 100 %. Conclusion The model of rat lung transplantation was established successfully with simple operation, which provided a basis for studying lung transplantation and immunology of lung graft.