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用作骨吸收研究的小鼠颅盖骨器官培养方法 被引量:5

InVitrocultureofNeonatalMousecalvariaasamodelforstudyofboneresorption.
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摘要 建立了新生小鼠颅盖骨器官培养方法,采用原子吸收分光光度技术测量培养上清液的钙含量,计算实验组/对照组的比值作为骨吸收指数。同时利用放射免疫分析方法测定上清液骨钙素含量,结合形态学观察,综合评价了培养颅盖骨的吸收活性,使颅盖骨吸收活性的检测更为准确可靠。 Weestablishedtheinvitroculturesystemofneonatalmousecalvaria.Boneresorp-tionwasdeterminedbythefolowingexaminations:(1)thereleaseofcalciumfromthecalvariaintothemediumwasmeasuredbyuseofatomicabsorptionspectrophotometer,andthetreatedtocontrolratiowascalculatedandusedasanindexofboneresorption;(2)theconcentrationofosteocalcinintheculturedmediumwasdeterminedbyradioimmunoasay;(3)histologicalexamination.Bycom-bineduseoftheexaminations,wewereabletoobjectivelydeterminetheboneresorptionofculturedcalvaria.
出处 《中华口腔医学杂志》 CAS CSCD 北大核心 1996年第1期48-50,共3页 Chinese Journal of Stomatology
关键词 颅盖骨 器官培养 骨钙素 骨吸收 口腔 CranialboneOrgancultureOsteocalcin
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  • 1孟昭亨,中华内分泌代谢杂志,1992年,8卷,41页

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