甘草甜素对小鼠朗格汉斯细胞的影响

The Effects of Glycyrrhizin on Langerhans' Cells of Mice

目的探讨甘草甜素对小鼠皮肤朗格汉斯细胞的影响。方法甘草甜素、生理盐水和地塞米松每日1次腹腔注射后在不同时间点获取耳部皮肤。用免疫组化技术检测小鼠表皮中的朗格汉斯细胞。结果①甘草甜素5 mg/kg、10 mg／kg及地塞米松1 mg／kg用药3 d后和1周后与用药0 d相比,朗格汉斯细胞密度显著降低。②甘草甜素80 mg/kg用药3 d后和1周后与用药0 d相比,朗格汉斯细胞密度显著升高。③用药4周后甘草甜素对朗格汉斯细胞密度的影响渐弱。结论甘草甜素对朗格汉斯细胞密度具有双相调节作用。低剂量使朗格汉斯细胞密度降低,高剂量使朗格汉斯细胞密度升高。

Objective To explore the effects of glycyrrhizin （GL） on Langerhans ceils （LCs） of mice. Methods The mice were treated intrapefitoneally with normal saline,different doses of GL and dexametha-sone, respectively, once daily. The mouse ear skin was excised at different times after treatment. The mouse LCs were detected by immunohistochemical technique after treatment, using rat IgG2a anti-mouse Ia monoclonal antibody. Results After 3 and 7 days of treatment, the densities of LC were significantly decreased in the groups treated with 5 mg/kg, 10 mg/kg of GL and 1 mg/kg of dexamethasone, but significantly increased in group treated with 80 mg/kg of GL. The effects of GL on the density of mouse LC weakened after 4 weeks of treatment. Conclusions The densities of mouse LC, decreased with low dose but increased with high dose of GL treatment, can be regulated in both directions by GL.