肝炎平抗兔血吸虫肝纤维化的实验研究

Experimental Study on Antifibrotic Effect of Ganyanping on Schistosomal Liver Fibrosis of Rabbits

目的探讨肝炎平对兔血吸虫肝纤维化血清中肝纤维化指标的影响。方法制备兔血吸虫肝纤维化模型。动物随机分为正常对照组(N组)、肝炎平治疗组(E组)及血吸虫肝纤维化模型组(D组)。对3组兔肝组织行病理学检查;采用放射免疫法对兔血清肝纤维化指标透明质酸(HA)、Ⅳ型胶原(ⅣC)、板层素(LN)及Ⅲ型前胶原(PCⅢ)进行检测。结果肝炎平组HA、ⅣC、LN及PCⅢ分别为(2484.3±384.9)、(1181.9±375.6)、(3791.8±1053.9)及(936.4±218.3)μg/L。模型组分别为(4823.1±969.2)、(1619.7±405.1)、(5425.9±1859.2)、(1250.0±403.6)μg/L。两组相应指标比较,差异有显著性意义(P<0.05)。结论肝炎平能降低血吸虫肝纤维化兔血清中的各项肝纤维化指标,有抗血吸虫肝纤维化的作用。

Objective To study the effect of Ganyanping on 4 serum indexes of liver fibrosis in rabbits with schistosomiasis. Methods The model of schistosomal liver fiboris in rabbits were established. The animals were randomly divided into 3 groups： normal control group （N）, Ganyanping treated group （E） and model group （D） . The pathological changes of livers were observed. The 4 serum indexes of liver fibrosis, including hyaluronic acid （HA）, collagen Ⅳ （Ⅳ-C）, laminin （LN） and precollagen Ⅲ （PCⅢ）, were evaluated by radioimmunoassay. Results The serum levels of HA, Ⅳ-C, LN and PCⅢ in Ganyanping treated group were （2484.3±384.9）μg/L, （1181.9±375.6）μg/L, （3791.8±1053.9）μg/L and （936.4±218.3）μg/L, respectively, and those in model group were （4 823.1±969.2）μg/L, （1619.7±405.1）μg/L, （5425.9±1859.2）μg/L and （1250.0±403.6）μg/L, respectively. There was significant difference in the 4 indexes of liver fibrosis between Ganyanping treated group and model group （P〈0.05） . Conclusion Ganyanping has the antifibrotie effect on sehistosomal liver fibrosis of rabbits by reducing the excessive collagen deposition and the degree of liver fibrosis quantified by serum HA, Ⅳ-C, LN and PCⅢ.