微球载体固定化碱性磷酸酶的底物／产物吸附现象

Adsorption of Immobilized Alkaline Phosphatase Latex to Substrate and Product

微球载体固定化碱性磷酸酶的底物／产物吸附现象史国利，马建标，何炳林（南开大学高分子化学研究所，天津，３０００７１）关键词碱性磷酸酶，固定化，底物，产物，吸附在酶固定化研究中载体对底物和产物的吸附会造成载体内部和载体周围底物和产物的浓度不均一［１］，从...

Aminated polystyrene latex beads were used in immobilization of Calf intestinal alkaline phosphatase.High adsorption of the immobilized-enzyme microbeads to substrate and product of enzyme,i.e.4-nitrophenyl phosphate(pNPP) and 4-nitrophenol(pNP) was found.The adsorption phenomenon had effect in determination of immobilized enzyme activity and the K_m value of immobilized enzyme.